ساراسادات نبوی زاده; محسن احمدی زارعی; کامبیز ایرجی; آیدا ایرجی; پروین قایم مقام; امید کوهی حسین آبادی; آیدا حسن پور; مریم مجاهد; مهشید جمشیدی; سجاد دانشی; نادر تنیده
Abstract
Background: Osteoarthritis is a major public health consideration which leads to disability because of chronic pain, stiffness, sleeping disorder and depression. Among the herbal products Psidium Guajava L. fruit could be regarded as a potential natural remedy due to its anti-oxidant characteristics. ...
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Background: Osteoarthritis is a major public health consideration which leads to disability because of chronic pain, stiffness, sleeping disorder and depression. Among the herbal products Psidium Guajava L. fruit could be regarded as a potential natural remedy due to its anti-oxidant characteristics. The aim of this study was to evaluate the healing effect of Psidium Guajava L. fruit on osteoarthritis of the knee in rats.Methods: Forty male Sprague-Dawley rats with induced osteoarthritis by intra-articular injection of 500U of type 2 collagenase in the left knee were recruited in four treatment groups (n=10): (A) control, (B) Piascledine (10mg/kg) as positive control, (C) oral administration of hydro-alcoholic extract of Psidium Guajava L. fruit (500mg/kg), and (D) oral administration hydro-alcoholic extract of Psidium Guajava L. fruit (1000mg/kg). After 8 weeks of daily administration, the outcome was evaluated by histopathological and radiographic assessments. The data were analyzed using Kruskal-Wallis nonparametric in SPSS version 23.00, and P<0.05 was considered statistically significant.Results: Psidium Guajava extract exhibited good radical scavenging activities with IC50 of 0.46 ± 0.13 mg/mL (quercetin as a positive control). Histopathological assessments of the cartilage showed smooth and continuous articular surface with columnar cell distribution in the experimental group that received 1000mg/kg Psidium Guajava L. fruit hydro-alcoholic extract which healed better than others, especially in the control group (p-value =0.02). The radiological assessments of the knee joints showed similar findings in histopathological assessment. Conclusion: Psidium Guajava L. fruit hydro-alcoholic extract might be regarded as an effective complementary and alternative treatment for osteoarthritis of the knee in rats.
giti Borzooeian; Nader Tanideh; Mehrzad Lotfi; Maral Mokhtari; Habibollah Nazem; Mahsa Sani; Mahin Salman-nezhad; omid koohi-hosseinabadi; sajad daneshi
Abstract
Background: Osteoarthritis is a progressive joint disease. Mastic fruit has antioxidant and anti-inflammatory properties. Bone marrow stem cells have the ability to differentiate into chondrocyte. The purpose of this study is evaluation the effect of Pistacia atlantica hydro alcoholic extract with bone ...
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Background: Osteoarthritis is a progressive joint disease. Mastic fruit has antioxidant and anti-inflammatory properties. Bone marrow stem cells have the ability to differentiate into chondrocyte. The purpose of this study is evaluation the effect of Pistacia atlantica hydro alcoholic extract with bone marrow- derived stem cells on induced knee osteoarthritis in rat.
Methods: Firstly, 50 male Wistar rats were divided into 5 groups of 10. Then, on days zero and 3, fifty μl collagenase type 2 under anesthesia was injected in the left knee. After 10 weeks, osteoarthritis was induced and rats based on the type of treatment were divided into 5 groups including: control, treatment by stem cell and Pistacia atlantica hydro alcoholic extract, Pistacia atlantica hydro alcoholic extract therapy, stem cell therapy and treatment with hyalgan. At the end of study, radiographic and histopathology surveys and also measured of antioxidant properties as MDA-TAC was done.
Results: After 12 weeks of treatment, radiographic and histopathological evaluation showed that the results of the cell with extract group was significantly different compared to other treatment groups and the control group (P <0.05). Also, based on biochemical evaluation of MDA-TAC in cell with extract group significant reduction in oxidative stress is observed compared to other treatment groups and control (P <0.05).
Conclusions: It seems that Pistacia atlantica hydro alcoholic extract with bone marrow- derived stem cells are effective in knee cartilage repair.
